南方医科大学学报2012，Vol.32Issue(2)：221-225,5.DOI:44-1627/R.20120209.1347.011
气管内滴入与雾化博莱霉素致小鼠肺纤维化模型的比较研究
Comparison of two mouse models of lung fibrosis induced by intratracheal instillation and intratracheal aerosol administration of bleomycin
摘要
Abstract
Objective To compare two methods of bleomycin administration for inducing lung fibrosis in mice. Methods Bleomycin (5 mg/kg) was intratracheally instilled or by intratracheal administration of its aerosol (100 ul) in mice. Evans Blue instead of bleomycin was intratracheally administered in the two manners to observe the liquid distribution in the lungs. On Days 14 after bleomycin treatment, the mice were sacrificed and the lungs were removed en bloc to determine the lung coefficient and hydroxyproline concentration, and the histological changes and lung fibrosis were evaluated by HE and Masson's trichrome staining, respectively. Results Evans Blue intratracheal aerosol distribution in the lung lobes. Intratracheal administration of bleomycin and its aerosol produced no obvious changes in the lung coefficient (P=0.434), and resulted in similar total histopathological scores and fibrosis level in mice (P=0.244 and 0.686, respectively). Bleomycin instillation caused obvious lung injury and lung fibrotic foci with significantly different histological and fibrotic scores between the pulmonary lobes (P=0.016 and 0.038, respectively), while bleomycin aerosol inhalation resulted in similar histological and fibrotic scores in the pulmonary lobes (P=0.466 and 0.29, respectively). A higher level of hydroxyproline was observed in mice with bleomycin aerosol administration (P=0.020). Conclusion Intratracheal bleomycin instillation and bleomycin aerosol inhalation induce similar levels of fibrosis in the lungs of mice, but the latter causes more extensive and more evenly distributed fibrosis in the lungs.关键词
肺纤维化/博莱霉素/气管内滴入/气管内雾化Key words
lung fibrosis/ bleomycin/ intratracheal aerosol inhalation/ intratracheally instillation分类
医药卫生引用本文复制引用
李伟峰,胡玉洁,袁伟锋,李理,黄文杰..气管内滴入与雾化博莱霉素致小鼠肺纤维化模型的比较研究[J].南方医科大学学报,2012,32(2):221-225,5.基金项目
广东省自然科学基金(S2011010000511) （S2011010000511）
广东省科技计划项目(2010B031600122) （2010B031600122）
