南方医科大学学报2012，Vol.32Issue(2)：234-238,5.DOI:44-1627/R.20120209.1216.008
高通量红细胞血型基因分型的多重连接依赖的探针扩增技术在—例抗Dia新生儿溶血病诊断中的应用
High-throughput genotyping multiplex ligation-dependent probe amplification for assisting diagnosis in a case of anti-Dia-induced severe hemolytic disease of the newborn
摘要
Abstract
Objective To report a rare case of hemolytic disease of the newborn (HDN) with kernicterus caused by anti-Di" diagnosed using high-throughput genotyping multiplex ligation-dependent probe amplification (MLPA). Methods Conventional serological methods were used to detect the antibodies related with HDN. The genotypes of more than 40 red blood cell antigens for the newborn and her parents were obtained using the high-throughput MLPA assay. The antibody titers were tested using a standard serological method. Results The unknown antibody against the low-frequency antigens was predicted based on the primary serological tests. The genotyping results for more than 40 red blood cell antigens of the newborn and her parents showed incompatible antigens of MNS and Diego blood group system, indicating the existence of anti-N or anti-Di1. Further serological tests confirmed anti-Di" existence in the plasma of the newborn and her mother. The titer of anti-Di' in the mother's plasma was 1: 32. Conclusion Severe HDN including kernicterus can result from anti-Dia. High-throughput genotyping MLPA assay can help type some rare antigens in complicated cases. The reagent red cell panels including Di'-positive cells are necessary in routine antibody screening test in Chinese population.关键词
红细胞血型/Diego血型系统/多重连接依赖的探针扩增技术/新生儿溶血病/核黄疸/抗DiaKey words
blood group of red blood cells/ Diego blood group system/ hemolytic disease of newborn/ kernicterus/ multiplex ligation-dependent probe amplification/ anti-Dia分类
医药卫生引用本文复制引用
姬艳丽,莫春妍,魏玲,周秀珍,张润青,赵阳,骆宏,王贞,罗广平..高通量红细胞血型基因分型的多重连接依赖的探针扩增技术在—例抗Dia新生儿溶血病诊断中的应用[J].南方医科大学学报,2012,32(2):234-238,5.基金项目
广东省自然科学基金(S2011040002949) （S2011040002949）
广州市医药卫生科技项目(201102A213230) （201102A213230）
广州市医药卫生科技项目(201102A212001) （201102A212001）
