南方医科大学学报2012，Vol.32Issue(3)：337-340,4.DOI:44-1627/R.20120308.0909.002
蟾酥注射液逆转HL60/阿霉素细胞多药耐药的机制
Mechanisms of Chansu Injection in reversing multidrug resistance of HL60/ADM cells
摘要
Abstract
Objective To investigate the mechanisms underlying the effect of Chansu Injection (CHS) in reversing multi-drug resistance (MDR) of HL60/ADM cells. Methods MTT assay was used to investigate the effect of CHS on adramycin (ADM) sensitivity of HL-60/ADM cells. Flow cytometry was used to observe the effect of CHS on the cell cycle of HL60/ADM cell. The expressions of NF-kB, MRP, GST-n, and iNOS were detected by immunocytochemistry. Results Treatment with CHS lowered the IC50 of ADM in HL60/ADM cells from 34.1971 umol/L to 17.4393 umol/L, and caused an increase in G0/G1, and G2/M phase cells with decreased S phase cells. CHS decreased the expressions of MRP mRNA and GST-π and MRP proteins but increased the expressions of iNOS and NF-kB proteins in the cells. Conclusion CHS can partly reverse MDR in HL60/ADM cells possibly by down-regulating MRP and GST-71, up-regulating NF-kB and iNOS, and promoting cell apoptosis, thereby increase ADM sensitivity of HL-60/ADM cells.关键词
蟾酥注射液/多药耐药/白血病/HL/阿霉素细胞Key words
Chansu Injection/ multi-drug resistance/ leukemia/ HL60 adramycin分类
医药卫生引用本文复制引用
熊新,周远大..蟾酥注射液逆转HL60/阿霉素细胞多药耐药的机制[J].南方医科大学学报,2012,32(3):337-340,4.基金项目
国家自然科学基金(81000067) （81000067）
