南方医科大学学报2012，Vol.32Issue(3)：349-353,5.DOI:44-1627/R.20120307.1728.028
CD133+人脐血造血祖细胞的干性维持培养及鉴定
Long-term culture and identification of CD133 + hematopoietic progenitor cells from human umbilical cord blood
摘要
Abstract
Objective To isolate CD133+ hematopoietic progenitor cells from human umbilical cord blood and optimize the culture condition for maintaining their stem cell characteristics. Methods CD133+ hematopoietic progenitor cells were isolated from human umbilical cord blood using magnetic cell sorting system, and the cells were detected by flow cytometry. Four methods were used for culturing cells. After 8 weeks' culture, cytomorphology, flow cytometry, immunocytochemistry and immunofluorescence assay were used to identify the characteristics of the stem cells. Results Over 80% of CD133 * hematopoietic progenitor cells were isolated from human umbilical cord blood using magnetic cell sorting system. The cells were effectively expanded using optimized serum-free medium after 8 weeks of cell culture, whereas the cells in other media differentiated into adherent cells in a poor state. Conclusion The optimized serum-free medium allows effective expansion of CD133+ hematopoietic progenitor cells that maintain stem cell characteristics after a long-term culture.关键词
人脐血/CD133+/造血祖细胞/干性/培养/鉴定Key words
human umbilical cord blood/ CD133+/ hematopoietic progenitor cells/ stem cell characteristics/ culture/ identification分类
医药卫生引用本文复制引用
吴小金,陈芳,陆滟霞,杨慧,彭璠莉,袁理,刘国炳,李学农..CD133+人脐血造血祖细胞的干性维持培养及鉴定[J].南方医科大学学报,2012,32(3):349-353,5.基金项目
国家自然科学基金(30871156) （30871156）
广东省科技计划项目(2009B030803041,2010B031600091) （2009B030803041,2010B031600091）
广东省自然科学基金(8151051501000057) （8151051501000057）
