南方医科大学学报2012，Vol.32Issue(3)：379-382,4.DOI:44-1627/R.20120308.0927.007
牛蒡子苷对晚期氧化蛋白产物诱导小鼠足细胞转分化的影响
Effect of arctiin on mouse podocyte epithelial-mesenchymal transition induced by advanced oxidation protein products
摘要
Abstract
Objective To study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition (EMT) induced by advanced oxidation protein products (AOPP). Methods Mouse podocytes were stimulated by 200 ug/ml AOPP for 24 h in the presence of 50, 100, 200, and 400 μmol/L arctiin. The expressions of o-smooth muscle actin, Grp78 and CHOP were detected using Western blotting. Result The expressions of a-SMA, Grp78 and CHOP were inhibited by arctiin, showing a dose-dependent effect within a given range of arctiin concentration. Conclusion AOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes, and arctiin can decrease EMT by alleviating the stress. This finding sheds light on a new scope of research of renal fibrosis.关键词
牛蒡子苷/足细胞/上皮-间充质转分化/晚期氧化蛋白产物Key words
arctiin/ mouse podocytes/ epithelial-mesenchymal transition/ advanced oxidation protein products分类
医药卫生引用本文复制引用
章俊,郭婷婷,杨蕾,杜庆生,华洁,刘蓉芝,汤珣..牛蒡子苷对晚期氧化蛋白产物诱导小鼠足细胞转分化的影响[J].南方医科大学学报,2012,32(3):379-382,4.基金项目
广东省自然科学基金(S2011010004053) （S2011010004053）
广州市科技计划重点项目(11C22120703) （11C22120703）
