南方医科大学学报2012，Vol.32Issue(3)：427-429,431,4.DOI:44-1627/R.20120307.1657.022
脑摄取动态平衡时不同麻醉深度丙泊酚对犬脑不同区域γ-氨基丁酸的影响
Effect of propofol at uptake equilibrium on γ-aminobutyric acid in different cerebral regions in dogs
摘要
Abstract
Objective To investigate the effect of propofol at doses for different anesthesia depths on y-aminobutyric acid (GABA) in different cerebral regions at propofol uptake equilibrium in dogs. Methods Twelve 12-18-month-old healthy hybrid dogs weighing 10-12 kg were randomly divided into light anesthesia group (n=6) and deep anesthesia group (n=6) with a single bolus dose of propofol (5.5 and 7.0 mg/kg, respectively) completed in 15 s followed by intravenous propofol infusion at a constant rate (55 and 70 mg ? kg-1 ? h-1, respectively). Blood samples (2 ml) were taken from the internal carotid artery and jugular vein to measure plasma propofol concentrations 50 min after the start of the infusion. The dogs were then sacrificed and tissues were taken from different brain regions and the cervical cord to measure GABA concentrations using high-pressure liquid chromatography (HPLC). Results The plasma propofol concentrations in internal carotid artery and jugular vein were similar in both light anesthesia group (3.00 ±0.31 and 3.10 ±0.51 ug/ml, respectively, P>0.05) and deep anesthesia group (6.41 ± 0.05 and 6.40 ± 0.11 fig/ml, respectively, P>0.05). GABA concentrations in the brain regions were significantly higher in deep anesthesia group than in light anesthesia group (P<0.05). The dorsal thalamus and hypothalamus showed greater GABA variations [(83.83±2.230%) and (85.83±1.72)%] compared to other brain regions at different anesthesia depths (P<0.05). Conclusions In both groups, plasma propofol concentrations in the internal carotid artery and internal jugular vein reach equilibrium at 50 min of propofol infusion. The variation of GABA is associated with the anesthesia depth of propofol, and GABA variation in the dorsal thalamus and hypothalamus plays an important role in propofol anesthesia.关键词
丙泊酚/γ-氨基丁酸/犬/脑/血浆浓度Key words
propofol/ y-aminobutyric acid/ dogs/ brain/ plasma concentration分类
医药卫生引用本文复制引用
汪燕,林春水,古妙宁,郭高锋,周枝凤,陈莺..脑摄取动态平衡时不同麻醉深度丙泊酚对犬脑不同区域γ-氨基丁酸的影响[J].南方医科大学学报,2012,32(3):427-429,431,4.基金项目
广东省自然科学基金(9151051501000054) （9151051501000054）
