分析测试学报2012，Vol.31Issue(3)：267-272,6.DOI:10.3969/j.issn.1004-4957.2012.03.005
丹酚酸B与牛血清白蛋白相互作用的电化学研究
Investigation on Electrochemical Behavior of Interaction between Salvianolic Acid B and Bovine Serum Albumin at DNA Modified Electrode
摘要
Abstract
The electrochemical behavior of interaction between salvianolic acid B ( SalB) and bovine serum album in ( BSA) was investigated by cyclic voltammetry and square wave voltammetry based on DNA-modified glass carbon electrode (GCE) in 0.01 mol/L phosphate buffer solution ( pH 7.4). SalB generated a sensitive oxidation peak of 0. 100 V at the DNA-modified GCE. After addition of BSA into the SalB solution, the oxidation current decreased and the peak potential shifted positively. The electrochemical parameters, such as the charge-transfer coefficient a and the standard electron-transfer rate constant ks were also changed. The binding number m of 1. 71 and the binding constant β of 1. 00 x 10 L/mol were calculated according to the data of the oxidation current, which indicated that SalB could combine with BSA to generate a non-electric complexes with combination ratio of 2 : 1. The results were in agreement with that provided by the fluorescence spectrometric method.关键词
丹酚酸B/牛血清白蛋白/DNA修饰电极/电化学法Key words
salvianolic acid B ( SalB ) / bovine serum albumin ( BSA ) / DNA modified electrode/ electrochemical method分类
化学化工引用本文复制引用
王月荣,刘馥婷,章弘扬,张敏,胡坪..丹酚酸B与牛血清白蛋白相互作用的电化学研究[J].分析测试学报,2012,31(3):267-272,6.基金项目
上海市科学技术委员会资助项目(10dz222050) （10dz222050）
