分析测试学报2012，Vol.31Issue(3)：307-311,5.DOI:10.3969/j.issn.1004-4957.2012.03.012
非水毛细管电泳法分离3种单磷酸腺苷其应用研究
Separation of Three Single Phosporic Acidic Adenosines by a Nonaqueous Capillary Electrophoresis Method and Its Application
宋娟梅 1成光 2边志明 3贾鹏1
作者信息
- 1. 淄博市环境监测站,山东淄博255040
- 2. 郑州大学化学系,河南郑州450052
- 3. 淄博建筑工程学校,山东淄博256400
- 折叠
摘要
Abstract
The separation of 2'-AMP, 3'-AMP and 5'-AMP were investigated by a nonaqueous capillary electrophoresis (NACE ) method. Effects of pH * value, nonaqueous solvent, different category buffer on the separation efficiency were studied. The optimum separation conditions are as follows; running medium; 200 mmol · L-1 Tris - H3BO3 containing 50% acetonitrile, pH * value; 10.0; applied electric field: 25 kV; column temperature; 25 t ; detection wavelength; 260 nm, and samples were injected from the cathodal end of the capillary by 50 mbar for 5 s. Under the optimal conditions, there are excellent linearities between peak heights and concentrations of the analytes in the concentration range of 1 -100 mg/L. The spiked recoveries ranged from 88% to 106% with RS-Ds less than 4% . The method was applied in the determination of adenosine samples with satisfactory results.关键词
非水毛细管电泳/单磷酸腺苷/核苷/分离Key words
nonaqueous capillary electrophoresis (NACE) / single phosporic acidic adenosine / nu-cleoside/ separation分类
化学化工引用本文复制引用
宋娟梅,成光,边志明,贾鹏..非水毛细管电泳法分离3种单磷酸腺苷其应用研究[J].分析测试学报,2012,31(3):307-311,5.
