分析测试学报2012，Vol.31Issue(3)：312-316,5.DOI:10.3969/j.issn.1004-4957.2012.03.013
T(4-Mop) PS4卟啉与牛血清蛋白相互作用的研究
Study on the Interaction of T(4-Mop)PS4 with BSA
摘要
Abstract
Using meso-terakis(4-methoxyl-3-sulfonatophenyl) porphyrin(T(4-Mop)PS4 ) as an electrochemical probe, a new electroanalytical method for the determination of bovine serum albumin (BSA) was established based on the interaction of BSA with T(4-Mop)PS4. A good linear relationship was obtained between the variation of peak current and concentration of BSA in the range of 2. 0 x 10-6 -1.0 ×l0-5 mol · L-1. The limit of detection ( LOD) was 1. 2 ×10-6 mol · L-1 and the relative standard deviation( RSD, n = 8 ) for 5. 0 × 10-6 mol · L-1 BSA was 2. 0% . The supramolecular complex of T(4-Mop)PS4 with BSA was studied by three different methods, including spectrophotom-etry, fluorometry and linear sweep voltammetric (LSV) methods. The binding ratio and binding constant of T(4-Mop)PS4 with BSA were obtained. The experimental data demonstrated that T(4-Mop) PS4 could form a 1 : 1 stable supramolecular complex with BSA, and hydrophobic interaction is the main reaction between them.关键词
卟啉/牛血清白蛋白/极谱法/结合常数/结合比/相互作用Key words
meso-terakis(4-methoxyl-3-sulfonatophenyl) porphyrin ( T( 4-Mop ) PS4) / bovine serum albumin ( BSA ) / polargraphy/ binding constant/ binding ratio/ interaction分类
化学化工引用本文复制引用
张红芬,郭玉晶,董川..T(4-Mop) PS4卟啉与牛血清蛋白相互作用的研究[J].分析测试学报,2012,31(3):312-316,5.基金项目
国家自然科学基金资助(20875059) （20875059）
