分析测试学报2012，Vol.31Issue(3)：347-350,4.DOI:10.3969/j.issn.1004-4957.2012.03.020
气相色谱-质谱法检测细菌中N-酰基高丝氨酸内酯类信号分子
Determination of N-Acyl-homoserine Lactones Signal Molecules by Gas Chromatography- Mass Spectrometry
摘要
Abstract
A rapid and sensitive method for the determination of N-acyl-homoserine lactones( AHLs) , a kind of quorum sensing signal molecules in Gram-negative bacteria, was established based on gas chromatography - mass spectrometric technique. The GC - MS conditions for the detection of AHLs were optimized. Under the optimal conditions, three kinds of AHLs, e. g. N-hexanoyl-L-homoserine lactone(C6 -HSL) , N-octanoyl-L-homoserine lactone(C8 -HSL) and N-tetradecanoyl-L-homoserine lactone(C14 - HSL) , were detected in the cultured supernatants of bacteria Pseudoalteromonas sp. NJ6 -3-1 under selected ion monitoring(SIM) mode. Compared with the traditional TLC-biosensor and HPLC method, this method is rapid and simple. AHLs in the samples could be detected qualitatively only by a simple sample handling procedure. The study provides a useful method in the detection of AHLs from complicated mixtures.关键词
N-酰基高丝氨酸内酯/气相色谱-质谱法/群体感应信号分子/细菌Key words
N-acyl-homoserine lactones/ gas chromatography - mass spectrometry ( GC - MS ) / quorum sensing signal molecule/ bacteria分类
化学化工引用本文复制引用
郭秀春,郑立,张魁英,徐鲁燕,王小如..气相色谱-质谱法检测细菌中N-酰基高丝氨酸内酯类信号分子[J].分析测试学报,2012,31(3):347-350,4.基金项目
国家自然科学基金面上项目(40776098) （40776098）
河南大学科研基金项目(B2011037) （B2011037）
