广东医学2012，Vol.33Issue(7)：883-886,4.
体外颗粒细胞培养中抗苗勒管激素与卵泡刺激素受体mRNA表达的相关性
Correlation between anti-Mullerian hormone and follicle-stimulating hormone receptor mRNA expression in granulosa cells in vitro.
摘要
Abstract
Objective To investigate the correlation between anti - Mullerian hormone (AMH) and follicle -stimulating hormone receptor (FSHR) mRNA expression in the cultured granulosa cells in vitro. Methods Kunming female mice were peritoneally injected with pregnant mare serum gonadotropin (PMSG) and sacrificed 48 to 54 hours after injection for collection of ovarian. Granulosa cells were isolated by mechanical method, trypsin digestion and low - speed centrifugation. Granulosa cells were diluted and incubated in DMEM/F12 medium containing 15% of fetal bovine serum at 37℃ in water - saturated environment of 5% CO2. Cell morphology, flow cytometry and immunofluoroscence were used for identification of ovarian granolosa cell. The primary granolosa cells were exposed to different concentrations of AMH. Real time - PCR was applied to analyze the FSHR mRNA expression in the ovarian granulosa cells. Results Cell purity was more than 80% . AMH induced concentration - dependent down - regulation of FSHR mRNA expression, and showed significant effect at 5 and 10 ng/mL. Conclusion granulosa cells can be obtained by mechanical method combined with trypsin digestion and brachytely centrifugation. FSHR staining provides quick and convenient approach in identifications of granulosa cells. AMH down -regulates FSHR mRNA in granulosa cells, suggesting a possible mechanism of pathogenesis of premature ovarian failure.关键词
颗粒细胞/抗苗勒氏管激素/卵泡刺激素受体/卵巢早衰Key words
granule cells/anti - mullerian hormone/follicular estrogen receptor mRNA expression引用本文复制引用
张迪,桑丽英,崔蓉,苗竹林,钟兴明,韦相才..体外颗粒细胞培养中抗苗勒管激素与卵泡刺激素受体mRNA表达的相关性[J].广东医学,2012,33(7):883-886,4.基金项目
广东省科技计划项目(编号:2009B030801270),广东省自然科学基金项目(编号:10151008010000000) （编号:2009B030801270）
