广东医学2012，Vol.33Issue(7)：894-896,3.
DNA甲基化在热休克诱导细胞周期素D1表达水平降低中的转录调控机制
Mechanism of DNA methylation in the regulation of heat shock-induced transcriptional inhibition of cyclin D1 expression.
摘要
Abstract
Objective To investigate the role of DNA methylation in heat shock -induced inhibition of human cyclin D1 expression. Methods The DNA methylation status and mutatan sites were detected by Methylmion Specific PCR ( MSP) and Stratagene kit, respectively. The effects of the methylation sites were evaluated by reporter gene analysis. Results Suggest that the + 65/67 sites of human cyclin Dl promoter were methylated under heat shock treatment. The heat shock response evaluated by reporter gene analysis was inverted when the putative methylation sites was mutated. Conclusion The heat shock - induced inhibition of cyclin Dl transcription involves the methylation of + 65/67 CpG sites.关键词
DNA甲基化/甲基化特异性PCR/热休克/细胞周期素D1Key words
DNA methylation/methylmion specific PCR/heat shock/cyclin Dl引用本文复制引用
郭志义,郝小惠,高雯雯,姜雪莲,赵丹丹,陈远,杨方..DNA甲基化在热休克诱导细胞周期素D1表达水平降低中的转录调控机制[J].广东医学,2012,33(7):894-896,3.基金项目
国家自然科学基金资助项目(编号:31050010),河北省高等学校科学研究计划项目(编号:2010154) （编号:31050010）
