广东医学2012，Vol.33Issue(7)：926-928,3.
广州地区献血人群 HIV-1gag基因序列分析
Gene analysis of HIV-1 gag gene in blood donors in Guangzhou.
摘要
Abstract
Objective To determine the sequence and subtype of HIV - 1 in blood donors in Guangzhou and its homogeneity with international reference strains. Methods HIV -1 RNA from HIV positive plasma in 32 blood donors, who was diagnosed as HIV - 1 patients with Western Blot, was amplified by RT - PCR. HIV - 1 gag gene was sequenced directly from the PCR product. Results There are 3 subtypes of HIV - 1 revealed. Among them 14 samples(43. 8% ) were CRF01_AE, in which 12 were closed to the international standard strain 01_AE. CN. 2005. 05GX156 with average gene distance of 0. 043 54; and 2 were closed to the international standard strain 01_AE. VN. 1998. 98VNBG6 with average gene distance of 0. 056 00. And 13 samples(40. 6% ) were CRF07_BC, and closed to the international standard strain 07_BC. CN. 2007. 07CNBJ550 with average gene distance of 0. 026 69. The other 5 samples( 15. 6% ) were 01B, and closed to the international standard strain 01B. CN. 2008. 08CYM003 with average gene distance of 0. 060 42. Conclusion The CRF01_AE and CRF07_BC subtypes of HIV - 1 accounts the major part in HIV - 1 infected blood donors in Guangzhou.关键词
人类免疫缺陷病毒-1/亚型/gag基因/献血者Key words
HIV-1/subtype/gag gene/blood donors引用本文复制引用
李玉笑,杜耀民,汪传喜,肖韶英,黄伯泉,梁浩坚,田也,杜荣松..广州地区献血人群 HIV-1gag基因序列分析[J].广东医学,2012,33(7):926-928,3.基金项目
广州市医药卫生科技计划项目(编号:2009-YB-134) （编号:2009-YB-134）
