广东医学2012，Vol.33Issue(8)：1114-1117,4.
气相色谱-质谱联用法测定尿液中N-乙酰天门冬氨酸
Determination of urine N-acetyl aspartic acid using gas chromatography-mass spectrometry
摘要
Abstract
Objective To evaluate the gas chromatography - mass spectrometry ( GC - MS) in quantitative detection of N - acetyl aspartic acid (NAA). Methods The urine samples were pretreated by urease methods and assessed by GC - MS. Two modes of GC - MS, SCAN and SIM were applied. SCAN was applied for assessment of retention time of chromatographic peak of resultants and their characteristic ion mass charge ratio, while SIM was selected for quantitative a-nalysis of NAA. Results There were two activated silylanize products after derivatization of NAA, NAAdi - TMS and NAAtri -TMS, with retention time of 17. 008 min and 17. 249 min, respectively. According to SIM, the linear correlation coefficient of NAA (1~1 000μg/mL) was 0. 998 4. At the levels of 10 μg/mL, 100 μg/mL and 1 000 μg/mL, intra -assay precisions in NAA detection were 2. 49% , 7. 61% and 7. 96% , respectively; while inter - assay precisions were 4. 28% , 8. 79% and 8. 1% , respectively; and recovery rates were 110% , 90. 03% and 108% , respectively; and deviations were 10% , -9. 97% and 8. 6% , respectively. The concentration of NAA in healthy control group was 8. 67 ±7. 88 μmol/mmol (mean±2SD) using GC -MS. Furthermore, the NAA in urine sample of Canavan disease (CD) offered by ERNDIM (2009-2010) were in 95% confidence interval. Conclusion GC - MS quantitative analysis is a rapid, sensitive and accurate method in urine NAA detection, providing laboratory evidence for screening and diagnosing CD.关键词
N-乙酰天门冬氨酸/气相色谱-质谱/Canavan病Key words
N - acetyl aspartic acid/gas chromatography - mass spectrometry/canavan disease引用本文复制引用
曾军,刘丽,彭敏芝,蔡燕娜..气相色谱-质谱联用法测定尿液中N-乙酰天门冬氨酸[J].广东医学,2012,33(8):1114-1117,4.基金项目
"十一五"国家科技支撑计划项目(编号:2006BAI05A07),广州市科学技术经费项目(编号:2010J-E231-1) （编号:2006BAI05A07）
