影像科学与光化学2012，Vol.30Issue(2)：102-109,8.
酸预处理对酶法骨明胶功能性质和分子质量分布的影响
Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process
摘要
Abstract
Gelatins were prepared from the bone pretreated using hydrochloride with various concentrations by protease-conditioned method in this article. The yields of protease-conditioned gelatin were decreased as the concentration of HC1 was increased from 0 to 4. 5% (mass fraction). The bloom values of protease-condition gelatin were all higher than 300 when the bovine bone were pretreated with HC1. Compared with commercial type B gelatin,protease-conditioned gelatin exhibited higher bloom strength,lower viscosity and higher IEP value. Compared with protein patterns of type B gelatin,different protein patterns of protease-conditioned gelatin were noticeable with the immersing of the new bands at the Mw of 165 Kda and 120 Kda. The IEPs of protease-conditioned gelatin were all in alkaline range which was related to the content of arginine. The results showed that the pretreatment process of bone was very important for protease-conditioned gelatin because the yield,viscosity and IEP were determined by the pretreatment condition.关键词
明胶/酶解法/酸预处理/等电点/分子量分布Key words
gelatin/protease-conditioned/acid pretreatment/IEP/protein patterns分类
化学化工引用本文复制引用
张兵,史京京,王颖,郭燕川,陈丽娟..酸预处理对酶法骨明胶功能性质和分子质量分布的影响[J].影像科学与光化学,2012,30(2):102-109,8.基金项目
中国科学院知识创新工程青年人才领域前沿项目(TYF0902). （TYF0902）
