中国妇幼健康研究2012，Vol.23Issue(2)：175-177,3.DOI:10.3969/j.issn.1673-5293.2012.02.014
液氮直投及程序冷冻冻存人卵巢组织的研究
Comparison of protocol of direct plunging into liquid nitrogen and slow controlled-rate freezing of human ovarian tissue
摘要
Abstract
Objective To compare the effect of direct plunging into liquid nitrogen and slow controlled-rate freezing of human ovarian tissue. Methods Cryopreservation of human ovarian tissue was accomplished by direct plunging into liquid nitrogen and slow controlled-rate freezing. Histological sections of fresh and frozen ovarian pieces ( primordial follicle, primary follicle and secondary follicle ) were detected by the method of hematoxylin-eosin staining. The thawed ovarian tissue pieces were cultured in vitro for 3 days, and then estradiol level was detected. Results In group of direct plunging into liquid nitrogen and group of slow controlled-rate freezing, the normal rate of primordial follicles morphous was 72. 96% and 81. 87% , respectively, and the difference was statistically significant (x2= 11. 27,P <0. 05 ). The normal rate of primary follicles morphous in two groups was 58. 62% and 34. 48% , respectively, and the difference was significant (x2 = 6.79 ,P <0. 05 ). The difference in estradiol level at 3rd and 6th day between two methods was not significant ( 108. 38 ± 9. 27 vs 105. 15 ± 9. 13,151.08 ±16.64 vs 147.38 ±20. 48, both P > 0.05 ). Conclusion Cryopreservation by protocol of direct plunging into liquid nitrogen and slow controlled-rate freezing is effective in preserving human ovarian tissue, and they have mild influence on ovarian tissue and function. But protocol of direct plunging into liquid nitrogen is more convenient and shortcut.关键词
人卵巢组织/液氮直投法/程序冷冻法/雌激素Key words
human ovarian tissue/ protocol of direct plunging into liquid nitrogen/ slow controlled-rate freezing/ estradiol分类
医药卫生引用本文复制引用
罗莉,杨继,赵静,韩曦,李东红,闫春芳,杨贵忠..液氮直投及程序冷冻冻存人卵巢组织的研究[J].中国妇幼健康研究,2012,23(2):175-177,3.基金项目
陕西省卫生厅科研课题资助项目(2010C08) （2010C08）
