南方农业学报2012，Vol.43Issue(1)：14-17,4.DOI:10.3969/j:issn.2095-1191.2012.01.14
基质辅助激光解吸电离飞行时间质谱检测蛋白质分子量的优化研究
Optimization of conditions for determination of protein molecular weight using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
摘要
Abstract
[Objective]The optimum conditions for measuring protein molecular weight were studied using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).[Method]Three different matrices,viz.,sinapic acid (SA),α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) were tested for determination of molecular weight of bovine serum albumin (BSA) and the effects of different sample concentration were observed on quantification of protein samples.[Result] The results demonstrated that 1 μg protein samples and sinapic acid (SA)matrix dissolved in water solvent (containing 0.1% trifluoroacetic acid and 50% acetonitrile to generate the concentration of 10 mg/mL) produced the best quality of MALDI-TOF-MS spectra.The sample required to determine the exact quantity was optimized as 500 ng.[Conclusion]This technique is convenient,rapid,sensitive and reliable for determining protein molecular weight.The optimal combination was determined to obtain optimum mass spectra,necessary for obtaining accurate molecular weight of highly complicated protein samples.关键词
基质辅助激光解吸电离飞行时间质谱/蛋白质/分子量/优化Key words
matrix assisted laser desorption ionization time of flight mass spectrometry/protein/molecular weight/optimization分类
生物科学引用本文复制引用
胡炜,付强,周洁,朱平川,蒙冰,张明..基质辅助激光解吸电离飞行时间质谱检测蛋白质分子量的优化研究[J].南方农业学报,2012,43(1):14-17,4.基金项目
Guangxi Natural Science Foundation(2011GXNSFB018028) （2011GXNSFB018028）
