南方农业学报2012，Vol.43Issue(1)：86-89,4.DOI:10.3969/j:issn.2095-1191.2012.01.86
大孔吸附树脂—比色法测定番茄水提物中的总皂苷
Determination of total saponin content in aqueous extract of tomatoes using macro-reticular resin colorimetric method
摘要
Abstract
[Objective]A method for determining total saponin content in aqueous extract of tomatoes was developed to facilitate studies on tomato saponins. [Method]After zymohydrolysis, tomato slurry obtained by smashing was subjected to D-101 macroporous resin to get crude saponins. Esculeoside A was used as reference and vanillin-perchloric acid was applied as chromogenic reagent to determine total saponin content at 550 nm. [ Result ]The standard curve of esculeoside A showed good linearity in the range of 0.052-0.312 mg with R2=0.9958, and the average recovery of added sample was 99.53% with 0.52%(n=6) RSD. Total saponin content obtained in five batches of crude saponin extraction was found to be 46.75±2.09%. [Conclusion]The method developed in the present study was rapid, accurate and easy to be used,therefore would be good for determination of total sapnion content in water extract of tomatoes.关键词
番茄水提物/总皂苷/大孔吸附树脂—比色法Key words
tomato water extract/ total saponin/ macro-reticular resin-colorimetric method分类
医药卫生引用本文复制引用
蒋海英,陈思呈,李典鹏..大孔吸附树脂—比色法测定番茄水提物中的总皂苷[J].南方农业学报,2012,43(1):86-89,4.基金项目
广西科学基金应用基础研究专项项目(桂科基0991020) （桂科基0991020）
桂林市科学研究与技术开发计划项目(20100103-3) （20100103-3）
