南方农业学报2012，Vol.43Issue(1)：99-102,4.DOI:10.3969/j:issn.2095-1191.2012.01.99
新型黄病毒在种鹅中垂直传播的研究
Vertical transmission of new type flavivirus in egg-laying goose
摘要
Abstract
[Objective]The present experiment was performed to investigate the vertical transmission of a new type flavivirus in egg-laying goose in order to provide essential epidemiological data for controlling flavivirus infection. [Method]The isolation of flavivirus and detection of its gene in different stages dead embryos, post-hatching eggshell wall and weak offspring samples of sick egg-laying goose were carried out using nest RT-PCR. [Result]Flavivirus was isolated and detected from all the above-mentioned samples, and the rate of flavivirus occurrence was recorded as 100.0, 39.6 and 45.0% in sick goose, dead embryos and post-hatching egg shell wall, respectively. The positive rate of flavivirus was 80.0% in weak offspring at 1 as well as at 15 days after hatching. [Conclusion]The flavivirus can be passed on to the next generation via embryo, I.e., the new type of flavivirus transmits vertically in egg-laying goose.关键词
鹅/黄病毒/巢式RT-PCR/垂直传播Key words
goose/ flavivirus/ nest RT-PCR/ vertical transmission分类
农业科技引用本文复制引用
赵冬敏,黄欣梅,刘宇卓,张敬峰,李银..新型黄病毒在种鹅中垂直传播的研究[J].南方农业学报,2012,43(1):99-102,4.基金项目
国家自然科学基金项目(31172345) （31172345）
江苏省农业科技自主创新资金项目[cx(11)4039] （11）
