南方农业学报2012，Vol.43Issue(1)：107-112,6.DOI:10.3969/j:issn.2095-1191.2012.01.107
黄颡鱼体表溃疡病病原菌分离鉴定及药敏试验
Isolation and identification of pathogenic bacteria from Pelteobagrus fulvidraco ulcerative syndrome and its drug sensitive test
摘要
Abstract
[Objective]The pathogenic bacteria were isolated and identified from ulcer diseased Pelteobagrus fulvidraco and drug sensitive tests were performed in order to develop effective control and prevention measures for disease. [Method] Pathogenic bacteria were extracted from the heart, liver and focus of nearly-dead Pelteobagrus fulvidraco, obtained from a farm in Yongfu County, Guangxi. Pathogenicity of the isolated strain was determined by artificial infection experiment. API 20NE biochemistry system was applied to identify pathogenic bacteria. Drug susceptibility test was conducted by using paper diffusion method. [Result]Four ulcer causing bacterial strains, viz., YFHS01, YFHS02, YFHS03 and YFHS04, found to possess strong pathogenicity to Pelteobagrus fulvidraco. The strain YFHS01 was Aeromonas sobria, while the rest were Aeromonas hydrophila. Four bacterial strains were insusceptible to penicillin and ampicillin, but highly susceptible to other 15 antibiotics, viz., ceftazidine, cephalosporinum, rocephin, gentamicin, neomycin, norfloxacin, enrofloxacin, ciprofloxacin, bacillosporin, levofloxacin, cefobid, tazocin, florfenieol, sarafloxacin hydrochloride and sul-fadiazine. [Conclusion]The above mentioned antibiotics may be used to control and prevent ulcer disease in Pelteobagrus fulvidraco caused by Aeromonas hydrophila and Aeromonas sobria in a farm of Yongfu County, Guangxi.关键词
黄颡鱼/溃疡病/嗜水气单胞菌/温和气单胞菌/分离鉴定/药敏试验Key words
Pelteobagrus fulvidraco/ ulcerative disease/ Aeromonas hydrophila/ Aeromonas sobria/ isolation and identification/ drug sensitive test分类
农业科技引用本文复制引用
黄钧,温华成,施金谷,黄艳华,梁正生,龙宜楠,秦纪璇..黄颡鱼体表溃疡病病原菌分离鉴定及药敏试验[J].南方农业学报,2012,43(1):107-112,6.基金项目
广西水产畜牧兽医局专项项目(桂渔牧财[2009]88号,桂渔牧财[2010]58号) （桂渔牧财[2009]88号,桂渔牧财[2010]58号）
