广西植物2012，Vol.32Issue(2)：226-230,5.DOI:10.3969/j.issn.1000-3142.2012.02.016
马铃薯遗传转化体系的优化及玉米淀粉分支酶基因SBEⅡb的导入
Optimization of genetic transformation system of potato and introduction of maize starch branching enzyme gene SBEⅡb into potatoFAN
摘要
Abstract
The highly effective potato transformation systems were established and optimized using tube potato plant-lets as transformation materials in this study. The maize starch branching enzyme gene(SBE Ⅱ b)was transformed into potato using Agrobacterium tumefaciens-mediated transformation method. 762 stem sections were infec ted by Agrobaterium-mediated tumefacien method. 4 transgenic plants were obtained by PCR measurement, implying that SEE Ⅱ b gene had been integrated into potato genome. It was also found that GUS gene could normal ly be expressed in stem sections and potato microtubers of the trasgenetic potato plants.关键词
玉米淀粉分支酶基因/马铃薯/遗传转化/淀粉Key words
maize starch branching enzyme gene/ potato/ genetic transformation/ starch分类
生物科学引用本文复制引用
范亚丽,阮颖,刘春林..马铃薯遗传转化体系的优化及玉米淀粉分支酶基因SBEⅡb的导入[J].广西植物,2012,32(2):226-230,5.基金项目
国家自然科学基金(31000132) （31000132）
阜阳师范学院校级自然科学基金(2010FSKJ12) （2010FSKJ12）
