华北农学报2011，Vol.26Issue(5)：29-33,5.
陆地棉Dirigent-like基因(GhDIR)的克隆与分析
Cloning and Analysis of Upland Cotton( Gossypium hirsutum )Dirigent-like Gene ( GhDIR )
摘要
Abstract
Dirigent-like gene encodes a protein invloved in the formation of lignin, and in defence to pathogen disease. Using an EST of a dirigent-like gene as the query probe to blast cotton EST database,a full-length 768 bp cDNA sequence of the dirigent-like gene was obtained and cloned by PCR, was named GhDIR. Sequence analysis indicated that it contained a complete open reading fragment( ORF) ,from 70 to 594 bp,encoding 175 amino acids, and has no intron. Sequence comparison and phylogenetic analysis showed the GhDIR cDNA was highly identical with its ortholog from Sea Island cotton, and the next most similar orthologs coming from other dicotyledons, but sharing low homology with monocotyledons. Quantitative PCR analysis showed that GhDIR was activated in roots of Upland cotton inoculated with Verticillium dahliae.关键词
陆地棉/电子克隆/DIR-like gene/表达分析/大丽轮枝菌Key words
Gossypium hirsutum/ In silica cloning /DIR-like gene/ Expression analysis/ Vertivillium dahlige分类
生物科学引用本文复制引用
赵付安,房卫平,杨晓杰,谢德意,李武,唐中杰..陆地棉Dirigent-like基因(GhDIR)的克隆与分析[J].华北农学报,2011,26(5):29-33,5.基金项目
国家转基因生物新品种培育科技重大专项(2008ZX08005-001) （2008ZX08005-001）
