华北农学报2011，Vol.26Issue(5)：107-111,5.
大豆MYB基因GmMYBJ7的克隆及表达分析
Cloning and Characterization of the MYB Gene GmMYBJ7 from Soybean
摘要
Abstract
The MYB proteins play an important role in plant, which involved in the regulation of the process of metabolism and development extensively. The function of MYB transcription factor gene GmMYBJ7,which was isolated from soybean using the method of RACE-PCR,was to be clarified preliminarily in this paper. The expression pattern of GmMYBJ7 in different organs was studied using Northen blot. The results showed that the expression of GmMYBJ7 was detected in the leaves and stems. The transcriptional activation ability of GmMYBJ7 protein was confirmed by the yeast system with p-galactosidase activity of 24. 31 U. Semi-quantitative RT-PCR analysis indicated that GmMYBJ7 may also repress the expression of some flavonoid biosynthetic genes, such as PAL ( Phenylalanine ammonia lyase) , C4H( cinnamate-4-hydroxylase) ,4CL(4-coumaroyl-CoA ligase) , CHS( Chalcone Synthase) ,CHI ( chalcone isomerase) and F3 H ( flavanone 3-hydroxylase) , which is considered to be related to flavonoid biosynthesis in paint.关键词
大豆/MYB转录因子/基因表达调控Key words
Soybean/ MYB transcription factors/ Gene expression and regulation分类
生物科学引用本文复制引用
杨文杰,吴燕民,唐益雄..大豆MYB基因GmMYBJ7的克隆及表达分析[J].华北农学报,2011,26(5):107-111,5.基金项目
中央级公益性科研院所科研业务费专项资金资助(2008-17) （2008-17）
