华北农学报2011，Vol.26Issue(5)：146-152,7.
CdCl2对牛卵母细胞体外成熟及凋亡的影响
The Influence of Bovine Oocytes in Vitro Maturation and Apoptosis by CdCl2
摘要
Abstract
In this research,bovine oocytes were dealed with different concentration of CdCl2(0,0. 8,1. 6,2.4 g/mL) ,we detect the change of oocyte mature rate,Ca2+ distribution, apoptosis rates, the mitochondrial membrane potential, Bcl-2 and Bax gene expression, this study lay the foundation for the further research that is the influence of heavy metal to female mammals germ cell development and cell apoptosis mechanisms. Bovine oocytes were treated with different concentration of CdCl2 (0,0.8,1.6,2.4 |I,g/mL) for 22 h, we count the rate of maturity and apoptosis; The early apoptosis of bovine oocytes was detected by Annexin V-FITC;The change of Ca + distribution and the mitochondrial membrane potential were respectively detected by Fluo-3/AM and Rhl23;Using RT-PCR,we detected the relative expression of Bcl-2 and Bax. The mature rate and apoptosis rate of the bovine oocytes by 0. 8 g/mL treatment group compared with control group was not significant (P＞0.05) ;1. 6μg/mL and 2.4μg/mL treatment group compared with control group were not significant (P＜0.05) ;with the increase of concentration of CdCl2,Ca2+ fluorescence intensity in cytoplasm was increasing,the mitochondrial membrane potential gradually declined, Bcl-2 and Bax Relative ratio was descending. The induction of oocytes apoptos is by CdCl2 is dose effect, which can restrain oocytes mature and promote cell apoptosis.关键词
CdCl2/牛卵母细胞/体外成熟/凋亡Key words
CdCl2/Bovine oocytes/In vitro maturation/ Apoptosis分类
农业科技引用本文复制引用
刘涛,李向臣,关伟军,马月辉..CdCl2对牛卵母细胞体外成熟及凋亡的影响[J].华北农学报,2011,26(5):146-152,7.基金项目
"863"高新技术发展计划(2006AA 10Z198) （2006AA 10Z198）
转基因生物新品种培育科技重大专项(2008ZX08009-003) （2008ZX08009-003）
国家科技支撑项目(2006BAD13 B08:2008 BADB2B01) （2006BAD13 B08:2008 BADB2B01）
