华北农学报2011，Vol.26Issue(5)：214-217,4.
cDNA-SRAP标记在玉米氮素营养诊断上的应用初探
Preliminary Application of cDNA-SRAP Marker in Diagnosis of Maize Nitrogen Nutritional Status
摘要
Abstract
To investigate the feasibility of diagnosis for nitrogen nutritional status of maize in molecular level, the elite hybrid variety Zhengdan958(Zea mays) was used to study the transcriptional difference of maize seedlings under different nitrogen levels by cDNA-SRAP markers. Several differentially expressed fragments were cloned and sequenced,and identified using Real-time PCR. The PCR amplification efficiency were various with different primer pairs. The nitrogen nutrition levels affected gene expression in maize. The results of cDNA-SRAP were basically consistent with those of Real-time PCR. Some fragments ( such as Nl, N9, N20, N30) could be taken as the candidate targets of molecular diagnosis of nitrogen nutrition in maize. cDNA-SRAP markers is an efficient way to develop molecular diagnosis of plant nutrition.关键词
玉米/氮素营养/cDNA-SRAP/分子诊断Key words
Maize(Zea mays) /Nitrogen nutrition/cDNA-SRAP/Molecular diagnosis分类
农业科技引用本文复制引用
高伟,苗利娟,黄冰艳,董保红,张新友..cDNA-SRAP标记在玉米氮素营养诊断上的应用初探[J].华北农学报,2011,26(5):214-217,4.基金项目
国家科技支撑计划重点项目(2008B AD4 B07-6) （2008B AD4 B07-6）
