华北农学报2011，Vol.26Issue(6)：20-26,7.
朝鲜碱茅△’-吡咯啉-5-羧酸合成酶(P5CS)基因的克隆及表达分析
Molecular Cloning and Expression Analysis of Delta 1-pyrroline-5-carboxylate Synthetase ( P5CS) Gene in Puccinellia chinampoensis
摘要
Abstract
Delta l-pyrroline-5-carboxylate synthetase( P5CS) plays a critical role in improving the stress tolerance of plants. In this study, the full length cDNA sequence of P5CS gene was cloned from Alkaligrass ( Puccinellia chinampoensis) leaves using RT-PCR method, the primers were designed according to the homologous P5CS gene sequences of other plant species. Sequence analysis showed that the nucleotide sequence of this gene is 2 158 bp,containing a complete open reading frame and encoding 716 amino acids,Genebank ;HQ637435. Nucleotide and amino acid sequence analysis revealed that PuP5CS shares high identity with the orthologs from Triticum aestivum. And its signal peptide, hydrophobicity/hydrophilic, trans-membrane domain, secondary structure and main functional domains were predicted. Semi-quantitative RT-PCR analysis showed that PuP5CS expressed in different tissues, but the expression in root was lower,and in leaf much higher. Various elevated levels of PuP5CS expression have been detected when exposed to 4 different stress experimental treatment, and the results was not the same.关键词
朝鲜碱茅/△’-吡咯啉-5-羧酸合成酶/基因克隆Key words
Puccinellia chinampoensis /P5CS/ Gene clone分类
生物科学引用本文复制引用
徐博,任伟,徐安凯,王志锋,孙启忠..朝鲜碱茅△’-吡咯啉-5-羧酸合成酶(P5CS)基因的克隆及表达分析[J].华北农学报,2011,26(6):20-26,7.基金项目
国家牧草产业技术体系 （）
