华北农学报2011，Vol.26Issue(6)：32-36,5.
陆地棉GhCYP1基因的分离及其表达分析
Isolation and Expression Pattern of GhCYP1 Gene in Upland Cotton
摘要
Abstract
In this study,based on the EST sequences of drought-resistant relative genes by employing SSH-PCR technique ,a full length 801 bp cDNA of Cyclophilin-like gene was cloned from cotton( Gossypium hirsutum) by using RACE technique, which containing a 522 bp coding sequence( CDS) ,and was designated as GhCYPl. Amino acid sequence alignment analysis showed that the GhCYPl was high identitied with cyclophilin from Arabidopsis thali-ana, Oryza sativa and Homo sapiens, all these cyclophilins containing a typical single-domain with a composition of 11 amino acids,three essential amino acids( R,F,H)for PPIase activity and a tryptophan( W) closely related to the site for cyclosporine A ( CsA) binding. Semi-quantitative PCR analysis showed that the GhCYPl was found with constitutive expression in the root, stem and leaf tissues, whereas the RNA transcriptional level in the root was highest, which suggested that this gene may be associated with uptaking water and transporting mineral, and played a pivotal role in the process of cotton adaptation to the drought environment.关键词
棉花/亲环蛋白/干旱胁迫/表达模式Key words
Cotton ( Gossypium hirsutum L. ) / Cyclophilin/ Drought-stress/ Expression pattern分类
生物科学引用本文复制引用
李元宝,王亦学,司怀军,吴家和..陆地棉GhCYP1基因的分离及其表达分析[J].华北农学报,2011,26(6):32-36,5.基金项目
国家转基因专项(2008ZX08010-004 （）
2009ZX08005-009B) （）
入侵昆虫综合技术研究与示范推广(201103026) （201103026）
