华北农学报2011，Vol.26Issue(6)：136-140,5.
HMC毒素诱导玉米同核异质B37原生质体细胞凋亡的检测
The Detection of Apoptosis in Protoplasts from B37 Homocaryotic Multicytoplasm Genotype Maize Induced by HMC-Toxin
摘要
Abstract
Apoptosis in protoplasts and leaves from Homokaryon maize B37 treated by HMC toxin were studied. The apoptotic features such as apoptotic bodies, chromatin margination or ring chromatin were found after the treatment stained by Hoechst33258. When treated by the same concentration of HMC toxin, the apoptosis rate of B37-C maize protoplasts were significantly higher than B37-N. In addition, the apoptosis rate was increased when the treatment time and concentration increasing. For the leaves of maize B37-C,the obvious DNA ladder was appeared with treatment concentration: 200 μg/mL; treatment time; 6 h. For the leaves of maize B37-N,the obvious DNA ladder was appeared with treatment concentration; 200 μg/mL; treatment time;9 h which was longer than leaves of maize B37-C. Apoptosis in protoplasts from Homokaryon maize B37 were same to the leaves. B37-C was more sensitive than B37-N.关键词
玉米小斑病菌C小种毒素/细胞凋亡/Hoechst33258染色/DNA梯状条带Key words
Helminthosporium maydis race C(HMC) toxin/ Apoptosis/Hoechst33258 /DNA ladder分类
农业科技引用本文复制引用
马春红,李秀丽,董文琦,及增发,李晓煜,戴志刚,王立安,贾银锁..HMC毒素诱导玉米同核异质B37原生质体细胞凋亡的检测[J].华北农学报,2011,26(6):136-140,5.基金项目
河北省自然科学基金项目(C2011301010) （C2011301010）
河北省人才工程培养经费(冀人社字[2010]353号) （冀人社字[2010]353号）
河北省科学技术研究与发展计划项目(10393912D) （10393912D）
科技部国际科技合作项目(2006DFB02480) （2006DFB02480）
农业部"948"项目(2008-Z20 （）
2011-G1) （）
