华北农学报2011，Vol.26Issue(6)：196-200,5.
仙人掌苷对荔枝霜疫霉菌的生物活性和蛋白组学分析
Bioassays and Proteomics Analysis of Peronophythora litchi Treated with Cacticin
摘要
Abstract
Bioassays showed that cacticin exhibited good antimicrobial activity and EC50 was 173. 52 μg/mL. To explore the protein-level antimicrobial mechanism of cacticin in Peronophythora litchi, the proteome of P. Litchi mycelium treated with cacticin was analyzed by two-dimensional electrophoresis(2-DE) and MALDI-TOF-MS. Results indicated sixteen protein spots showed significant and reproducible changes after cacticin treatment, among which e-leven were down-regulated, four were up-regulated, and one was newly induced by cacticin. Five successfully identified proteins were assigned as mitochondrial dicarboxylate carrier protein ( DIC 1) ,cysteine synthetase A, ATP-binding cassette transporters ( ABCF-EF-3 ) , class Ⅲ stress response-related ATPase and hypothetical protein BSU31030. These proteins were involved in drug stress response, amino acid and sulfur metabolism, membrane transportation and discharge, and energy metabolism. Altogether, the results provided important information on the molecular mechanisms of antimicrobial activity of cacticin against P. Litchi.关键词
仙人掌苷/荔枝霜疫霉菌/抑菌活性/蛋白组Key words
Cacticin/Peronophythora litchi/Antimicrobial activity/Proteome分类
生物科学引用本文复制引用
赖多,曹永军,卢梦玲,徐汉虹..仙人掌苷对荔枝霜疫霉菌的生物活性和蛋白组学分析[J].华北农学报,2011,26(6):196-200,5.基金项目
国家自然科学基金项目(30571235) （30571235）
广东省农业领域重点项目-农业公关"安全农业投人品开发"(2009 A020101003) （2009 A020101003）
