华北农学报2011，Vol.26Issue(6)：207-211,5.
抗感水稻品种受灰飞虱为害后叶片活性氧及超微结构的变化
Changes in Reactive Oxygen Species and Ultrastructure in Resistant and Susceptible Rice Leaves Infested by Small Brown Planthopper
摘要
Abstract
The small brown planthopper( SBPH) ,Laodelphax striatellus Fallen( Homoptera: Delphacide) , is a serious pest in rice( Oryza sativa L. ) . Diaminobenzidine( DAB) hydrogen peroxide( H2O2) staining of rice leaf infested by SBPH indicated pest response was earlier in resistant rice than that in the susceptible. Accumulation of H2O2 was detected in the resistant variety infested by SBPH for 3 days but not in the susceptible rice. The H2O2 content declined gradually to the initial level in resistant rice cells but tended to increase in the susceptible rice with the damage extended. Observation with scanning electron microscope( SEM) on epidermis of affected rice leaves indicated that wound healing likely caused by some substance secreted by rice was found in the resistant variety and then protected the infected seedling but not existed in the susceptible. Observation with(TEM) on ultrastructure of rice leaf cells showed the chloroplast structure was integrate, Whose grana thylakoid and lamellar structure were intact in resistant rice but disordered arrangement of the internal chloroplast structure appeared in the susceptible variety infested by SBPH for 3 days. Chloroplast membrane structure dissolved in both tested rice and was loose and disordered in susceptible rice but lamellar structure still existed and arranged orderly when the tested rice seedling was infested for 6 days.关键词
水稻/灰飞虱/活性氧/超微结构Key words
Rice/ Small brown planthopper/ Reactive oxygen species/ Ultrastructure分类
农业科技引用本文复制引用
段灿星,彭高松,朱振东,李洪杰,王晓鸣..抗感水稻品种受灰飞虱为害后叶片活性氧及超微结构的变化[J].华北农学报,2011,26(6):207-211,5.基金项目
国家自然科学基金(30971746) （30971746）
转基因生物新品种培育科技重大专项(2009ZX08009-046B) （2009ZX08009-046B）
