华北农学报2011，Vol.26Issue(z1)：5-8,4.
人源白细胞介素(h-IL2)基因克隆及原核表达的研究
Study on Gene Cloning and Prokaryotic Expression of Human Interleukin-2
刘钦松 1张丛丛 1刘孟刚 1许彤1
作者信息
- 1. 山东博奥克生物科技有限公司,山东聊城252000
- 折叠
摘要
Abstract
To construct a recombinant espression vector of human Interleukin-2, and eapress in prokaryotic cells, which is helpful for the further purification of recombinant proteins and antibodies production. Methods; Am plify the h-JL2 gene by PCR,the DNA fragments were cloned into pMD-18T simple vectors,transformed into compe tent DH5a,recombinant plasmid PCR,restriction enzyme digest and sequencing confirmed that the fragment was h IL2 and the sequence was identical to that published in GenBank. (NM_000586. 3). Then the h-IL2 gene were cloned into pGEX-4T-l constructing expression vectors, transformed into competent BL21(DE3) , induced by IPTG, SDS-PAGE verification successful expression of the fusion protein GST-IL2.关键词
白细胞介素/基因克隆/原核表达Key words
Human Interleukin/Gene cloning/Prokaryotic expression分类
生物科学引用本文复制引用
刘钦松,张丛丛,刘孟刚,许彤..人源白细胞介素(h-IL2)基因克隆及原核表达的研究[J].华北农学报,2011,26(z1):5-8,4.
