华北农学报2012，Vol.27Issue(1)：63-67,5.
NaCl筛选法获得无标记耐盐转基因水稻的高效转化技术研究
Study on NaCl Screening to Obtain Marker-free and High Effective Salt-tolerant Transgenic Rice
摘要
Abstract
It is an effective method to use NaCl selecting to generate marker-free salt-tolerant,transgenic rice. We used the three japonica rice cultivar( ZhongHua11 ,Xiushui11 , Chunjiang06) mature embryos callus as materials, SKC1 as the target gene to study that on the conditions of screening NaCl, heat shock treatment and liquid co-culture of Agrobacterium-mediated gene transfer salt-tolerant rice efficiency. The results showed that heat shock and liquid co-culture of rice could enhance the efficiency of salt tolerance gene transfer. Among them, the heat shock treatment increased the maximum efficiency of gene transfer was 46. 2% , while the liquid co-culture increased the highest efficiency of gene transfer was 60. 7% .关键词
水稻/NaCl筛选/热激/液体共培养/转化效率Key words
Rice/ NaCl screening/ Heat shock/ Liquid co-culture/ The efficiency of gene transfer分类
农业科技引用本文复制引用
赵潇,罗园园,赵艳..NaCl筛选法获得无标记耐盐转基因水稻的高效转化技术研究[J].华北农学报,2012,27(1):63-67,5.基金项目
国家自然科学基因资助项目(30871511 （）
30771317) （）
农业部转基因生物新品种培育重大专项(2008ZX0810-003 （）
2009ZX08001-022B) （）
同家"863"计划资助项目(2006AA10Z1A9) （2006AA10Z1A9）
中国博士后基因资助项目(20090450477) （20090450477）
