华北农学报2012，Vol.27Issue(1)：93-97,5.
36个马铃薯品种的SSR分析
SSR Analysis on 36 Potato Varieties
摘要
Abstract
To know the genetic differences in DNA level among 36 potato varieties, the polymorphism among the tested plants were analyzed based on SSR molecular marker. 10 primer combinations, which suited the genome DNAs of 36 potato varieties, were selected from the 90 primer combinations. 301 bands of SSR markers were amplified , the percentage of polymorphism bands reached 71. 1 % . The differences in SSR fingerprints amplified with primers STACCA3 and SSR111 were significant. It can be used as molecular evidence to distinguish 36 potato varieties. The genetic distance ( GD) of 36 tested plants ranged from 0.33 to 0.85, and the average of genetic distance was 0. 54. There were 20 varieties,GD of which was greater than the average GD,accounting for 55. 56% of the varieties tested. Using the GD value of 0.57 as threshold,36 tested plants could be clustered into 7 groups,the genetic relationships among tested varieties were revealed at DNA molecular level.关键词
马铃薯/品种/SSR分析/遗传差异Key words
Potato/ Varieties/ SSR analysis/ Genetic difference分类
农业科技引用本文复制引用
张自强,于肖夏,鞠天华,马艳红,于卓,蒙美莲,李长青..36个马铃薯品种的SSR分析[J].华北农学报,2012,27(1):93-97,5.基金项目
"十二五"国家科技支撑计划课题(2012BAD02B05) （2012BAD02B05）
内蒙古科技创新引导奖励资金项目(2011) （2011）
国家现代农业产业(马铃薯)技术体系建设专项资金项目(nyeytx-15 （马铃薯）
gwzj-21) （）
呼和浩特市农业综合开发科技创新项目(2011-计-2) （2011-计-2）
