华南农业大学学报2012，Vol.33Issue(2)：175-177,3.
一种测定龙眼果柄离层纤维素酶活性的方法
An Assay Method for Cellulase Activity in Longan Pedicle Abscission Layer
摘要
Abstract
A new determination method of cellulase activity-gel diffusion-tissue blot was described. This paper provided the basic principle, operation procedures and calculation methods for cellulase assay, and applied this method to analyze the activity of cellulase in the abscission layer in longan pedicle during fruit abscission. The results showed that this method had advantages such as less material requirement, high sensitivity, good reproducibility, simple operation, and saving time and labor, and it is very suitable for the determination of cellulase activity in abscission layer.关键词
纤维素酶/离层/凝胶扩散-组织印迹法Key words
cellulose/ abscission layer/ gel diffusion-tissue blot method分类
农业科技引用本文复制引用
杨子琴,李建国,王惠聪,黄旭明..一种测定龙眼果柄离层纤维素酶活性的方法[J].华南农业大学学报,2012,33(2):175-177,3.基金项目
国家自然科学基金(30871693,31171919) （30871693,31171919）
国家荔枝龙眼产业技术体系果实发育与调控岗位(CARS-33-11) （CARS-33-11）
