华南农业大学学报2012，Vol.33Issue(2)：239-243,5.
高效液相色谱法测定牛奶及牛可食性组织中咪唑苯脲
Determination of Imidocarb Residues in Bovine Edible Tissues by High-Performance Liquid Chromatography
摘要
Abstract
A confirmative method has been developed to determine imidocarb (IMD) residues in edible tissues and milk of bovine by high-performance liquid chromatography( HPLC). The tissues and milk were first hydrolyzed by β-glucosidase. The tissues were extracted with hydrochloric acid, ultrasonicated and back-extracted with extract while the milk was extracted with extract only. The mobile phase was acetoni-trile and 1 -pentansulfonic acid sodium salt solution. The analyte was detected by UV absorptive spectros-copy after separation by C18 column. The range of linearity was 0. 01 - 10. 00 μg/mL; the relevant coefficient was more than 0. 999. The limit of detection ( LOD) was 0. 01 μg/g, and the limit of qualification (LOQ) was 0. 02 μg/ g. Mean recoveries from bovine tissues and milk fortified at LOQ, MRL( the maximum residue limit) and 2MRL were from 80. 02% -114. 72% , whereas the corresponding RSDs were from 0. 83% - 12. 69%. In conclusion, the established method is simple, sensitive and reproducible for the determination of imidocarb residues in edible tissues and milk of bovine.关键词
高效液相色谱/咪唑苯脲/残留/牛奶/牛可食性组织Key words
high-performance liquid chromatography/ imidocarb/ residue/ milk/ edible tissue of bovine分类
农业科技引用本文复制引用
于慧敏,黄显会,杨刚,方秋华..高效液相色谱法测定牛奶及牛可食性组织中咪唑苯脲[J].华南农业大学学报,2012,33(2):239-243,5.基金项目
2009年农业行业标准制修订项目 （）
