华南农业大学学报2012，Vol.33Issue(2)：248-252,5.
喹烯酮及其主要代谢物在猪体内的残留规律研究
Study on Residue Depletion of Quinocetone and Its Major Metabolites in Swine
摘要
Abstract
Residue depletion of quinocetone and three of its major metabolites in muscle, liver, kidney and fat of swine were determined in this study. Quinocetone was administered to 40 healthy cross-bread swine orally at dosage of 20 mg · Kg-1 body mass twice a day for 5 consecutive days. Animals were slaughtered in groups and samples were taken at different time intervals after administration. The tissues were extracted with ethyl acetate, and cleaned up with HLB SPE column. A sensitive high-performance liquid chromatography-tandem mass spectrometry ( LC-MS/MS) method was developed to determine quinocetone and its metabolites. Quinocentone and its metabolites were eliminated more slowly in swine muscle than those in other tissues. The highest drug levels occurred in liver for 3 -methyl-styryl ketone-quinoxaline. The residue lasted the longest half-life of elimination for 39. 50 h with 3-methyl-styryl ke-tone-quinoxaline -N4-monoxide in pig muscle. The 3-methyl-2-carboxylic acid-quinoxaline was detected only in kidney at very low concentration.关键词
喹烯酮/代谢物/高效液相色谱串联质谱法/残留消除/猪Key words
quinocetone/ metabolites/ high-performance liquid chromatography-tandem mass spectrometry/ residue depletion/ swine分类
农业科技引用本文复制引用
钟家林,王林,赵宁,于慧敏,贾慧琴,鲁晓雄,吴子莹,丁焕中..喹烯酮及其主要代谢物在猪体内的残留规律研究[J].华南农业大学学报,2012,33(2):248-252,5.基金项目
国家重点基础研究发展计划(973计划)(2009CB118805) （973计划）
“十一五”国家科技支撑计划项目(2009BADB7B05-03). （2009BADB7B05-03）
