华南农业大学学报2012，Vol.33Issue(2)：256-258,263,4.
猪繁殖与呼吸综合征病毒ORF5/6/7基因真核载体的构建
Construction of Eukaryotic Expressing Vector from ORF5/6/7 of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
Specific primers aiming to ORF5-ORF7 of porcine reproductive and respiratory syndrome (PRRSV) were designed. The target segments with restricted enzyme digestive sites were obtained by RT-PCR with PRRSV GD-XH and GD08-1 as template. All the segments were inserted into vector Peg-FP-N1 to obtain six recombinant plasmids:Pegfp-Nl-GD-XH-GP5, Pegfp-Nl-GD-XH-M, Pegfp-Nl-GD-XH-N, PEGFP-N1-GD08-1-GP5, PEGFP-N1-GD08-1-M, Pegfp-Nl-GD08-l-N. The recombinant plasmid was transfected into Marc-145 cell. Fluorescence microscopy showed that EGFP were expressed in all transfected cells.关键词
猪繁殖与呼吸综合征病毒/ORF5/6/7/pEGFP-N1Key words
porcine reproductive and respiratory syndrome virus/ ORF5/6/7/ pEGFP-Nl分类
农业科技引用本文复制引用
康艳梅,赵明秋,沈海燕,琚春梅,陈金顶..猪繁殖与呼吸综合征病毒ORF5/6/7基因真核载体的构建[J].华南农业大学学报,2012,33(2):256-258,263,4.基金项目
广东省科技计划项目(2007 A020300064) （2007 A020300064）
广东省自然科学基金创新团队项目(5200638) （5200638）
教育部“长江学者和创新团队发展计划”创新团队项目(TRT0723) （TRT0723）
