渔业科学进展2012，Vol.33Issue(1)：61-65,5.
对虾白斑综合征病毒VP28酵母表面展示
Yeast surface display of white spot syndrome virus VP28
摘要
Abstract
pYDl was selected to make a vaccine using Saccharomyces cerevisiae pair as the live carrier. One pair of primers was designed according to the gene encoding white spot syndrome virus (WSSV) of VP28. VP28 gene was obtained by using PCR method. Fragment of VP28 was ligated into pYDl and then transformed with E. Coli TOP10. The construct was propagated in E. Coli TOP10 and then was transformed into the yeast strain EBY100. The display of VP28 protein on the yeast surface was confirmed by fluorescent staining with antibody a-nalysis. The results showed that VP28 was displayed on yeast surface. The infection test indicated that the yeast cells displaying VP28 was safe to Procambarus clarkii. This work is helpful for further research on the immunological effect of the live vaccine.关键词
对虾白斑综合征病毒/VP28/酵母表面展示/安全性Key words
WSSV/VP28/Yeast surface display/Safety分类
农业科技引用本文复制引用
李新新,刘庆慧,张秀丽,黄倢..对虾白斑综合征病毒VP28酵母表面展示[J].渔业科学进展,2012,33(1):61-65,5.基金项目
对虾行业专项(200803012)和国家自然科学基金(30871942)共同资助 （200803012）
