家畜生态学报2012，Vol.33Issue(1)：14-18,5.
过氧化物酶体增殖激活受体α(PPARα)基因突变对牛的背膘厚和胴体长的影响
A Mutation in PPARα Gene Impact Backfat Thickness and Carcass Length in Cattle
摘要
Abstract
To reveal the SNP of PPARa gene exon7 and evaluate its relationship with carcass traits and meat traits in cattle, a total of 717 individuals from six breeds (QC, NY, JX, LX, XN and Angus) were genotyped by PCR-SSCP and DNA sequencing in PPARa gene exon7 and analyzed its correlation of the SNP with some carcass traits and meat traits in 108 Qinchuan cattle. C→T mutation was detected at 184bp in PPARa gene exon7. Allele frequencies of PPARa-E/F in the six breeds were 0. 225/0. 775, 0.151/0.849, 0.125/0.875, 0.123/0.877, 0.070/0.930 and 0. 157/0.783 for NY, QC, JX, Angus, LX and XN, respectively. Except for NY breed in moderate polymorphisms (PIC=0. 288), other five breeds were in low genetic diversity in the locus(PIC<0. 25). Correlation analysis showed that the mutation had significantly related to back fat thickness and carcass length in QC breed. Animals with the genotype FF had significantly higher traits than these with genotypes EE and EF in back fat thickness trait (P<0. 05), and animals with the genotype FF had much higher than those with genotype EE (P<0. 05) in carcass length trait. In conclusion, this locus is likely to be a major QTN or tightly linked that affect production traits, it can be a candidate molecular marker for the beef cattle breeding.关键词
过氧化物酶体增殖激活受体α (PPARα)基因/SNPs/肉质性状/分子标记Key words
PPARa gene /SNPs/ meat traits/molecular marker分类
农业科技引用本文复制引用
樊月圆,富国文,昝林森,付常振,李卫真,王绍卿..过氧化物酶体增殖激活受体α(PPARα)基因突变对牛的背膘厚和胴体长的影响[J].家畜生态学报,2012,33(1):14-18,5.基金项目
教育部创新团队(IRT0940) （IRT0940）
国家转基因重大专项(2008ZX08007-002) （2008ZX08007-002）
陕西省“13115”科技创新工程(2010ZDGC-01) （2010ZDGC-01）
