基础医学与临床2012，Vol.32Issue(4)：338-341,4.
错配修复基因hMLH1启动子萤光素酶报告基因载体的构建与鉴定
Construction and identification of luciferase reporter gene vector containing hMLHl promoter
摘要
Abstract
Objective To construct luciferase reporter gene vector containing human MLH1 promoter and to assay the transcriptional activity of hMLHl promoter induced by E2. Methods hMLHl promoter ( -1 953/+53) were amplified from the genomic DNA of human by PCR and cloned into luciferase reporter gene vector, pGL3-Basic. The recombined vector was transfected into HEK293 cells, and the activity of the luciferase was determined after simulation by E2. Results The results of restriction enzyme digestion and sequencing indicated that the recombi-nant vector pGL3-Promoterl-luc was successfully constructed. After transcription of pGL3-Promoterl -luc, the activity fold of the luciferase was 7. 45 ±0. 81 induced by E2, which is significantly higher than 3. 28 ±0. 19 without E2 (n=3,F＜0. 001). Conclusions The hMLHl promoter contains the regulatory sequence associated with E2.关键词
错配修复基因/hMLH1/启动子/雌激素/双萤光素酶报告基因Key words
mismatch repair gene/ hMLHl/ promoter/ estrogen/ dual-luciferase reporter gene分类
医药卫生引用本文复制引用
卢俊宇,金鹏,高巍,陆晓娟,王亚婷,盛剑秋..错配修复基因hMLH1启动子萤光素酶报告基因载体的构建与鉴定[J].基础医学与临床,2012,32(4):338-341,4.基金项目
国家自然科学基金(81072041) （81072041）
医学分子生物学国家重点实验室专项经费(2060204) （2060204）
