基础医学与临床2012，Vol.32Issue(4)：346-349,4.
敲低错配修复基因hMLH1可导致人胚肾细胞株293t的微卫星不稳定
Knocking down mismatch repair gene hMLH1 may influence microsatillite stability in human embryonic kidney cell line 293t
摘要
Abstract
Objective To explore the ralations between expression of hMLHl gene and microsatillites stability in human embryonic kidney cell line 293t. Methods A plasmid vector encoding shRNA targeting hMLHl (pGPU6/GFP/ Neo-shMLHl) was constructed for knocking down hMLHl gene in 293t cell line,and evaluated the microsatallite stability before and after knocking down. Results The expression of MLH1 in knocking down group was obviously knocked down in 293t cell, and the microsatallite states was changed from microsatallite stability into microsatallite instability. Conclusions Knocking down hMLHl gene may lead to microsatallite instability in cell line 293t.关键词
错配修复基因/hMLH1/微卫星不稳定/RNA干扰Key words
mismatch repair gene/ hMLHll/ microsatallite instability/ RNA interference分类
医药卫生引用本文复制引用
高巍,金鹏,陆晓娟,卢俊宇,王亚婷,盛剑秋..敲低错配修复基因hMLH1可导致人胚肾细胞株293t的微卫星不稳定[J].基础医学与临床,2012,32(4):346-349,4.基金项目
国家自然科学基金(81072041) （81072041）
医学分子生物学国家重点实验室专项经费(2060204) （2060204）
