基础医学与临床2012，Vol.32Issue(4)：354-358,5.
P53 shRNA对肝癌细胞增殖的影响
Effect of P53 shRNA on proliferation of hepatoma cell
摘要
Abstract
Objective To observe the effect of P53 shRNA on the proliferation and expression of P21 protein in SMMC-7721 cell line. Methods Design short hairpin RNA(shRNA) sequences targeting human wild type P53 gene and construct recombinant plasmid pGPU6/GFP/Neo-P55 shRNA. SMMC-7721 cells were transfected with the recombinant plasmid and negative control vector respectively. Stably expressing clones were selected with G418. The proliferation was detected with clone form assay. The expression of P53 and P21 mRNA and protein were detected by RT-PCR and Western blot, respectively. SMMC-7721 cells were transplanted by subcutaneous injection to form transplantation tumor in nude mice. Then pGPU6/GFP/Neo-P53 shRNA or negative control shRNA were injected into implanted tumors directly, and tumor volume and weight were detected. Results P53 shRNAs, including PI, P2 and P3 , can severely decrease P53 mRNA levels (P＜0. 05 , P＜0. 05 or P＜0. 01) . The clone form efficiency was increased significantly after interfering P53 function in SMMC-7721 cells(P＜0. 05) , and the expression of P21 mRNA and protein were obviously decreased following the interference of P53. After P53 was silenced, tumor volume and weight were significantly increased (P＜0. 05). Conclusions P53 shRNA silencing P53 to lower P21 protein expression may make SMMC-7721 hepatic carcinomas cells malignant proliferation.关键词
RNA干扰/P53/P21/肝癌Key words
RNA interference/ P53/ P21/ hepatoma分类
医药卫生引用本文复制引用
欧贤红,廖柳凤,陆游,徐恒,刘华钢..P53 shRNA对肝癌细胞增殖的影响[J].基础医学与临床,2012,32(4):354-358,5.基金项目
国家自然科学基金(30672363,30950028),广西研究生教育创新计划(2009105981007D25) （30672363,30950028）
