基础医学与临床2012，Vol.32Issue(4)：363-368,6.
Islet-1在乙酰化调控网络中特异性辅助C3H10T1/2细胞向心肌样细胞分化
Islet-1 specifically assists the process of C3H10TI/2 cell differentiating into cardiomyocyte-like cells in the histone acetylation regulatory network
摘要
Abstract
Objective To find out which protein domains of histone acetylase ( HATs) and histone deacetylase ( HDACs) interact with Islet-1 during the differentiation of C3H10T1/2 cells transfected with Islet-1 lentiviral vector into cardiomyocyte-like cells, and to prove the pivotal role of Islet-1 in the network of histone acetylation. Methods Culture and observe C3H10T1/2 cells transfected with Islet-1 lentiviral vector. The position of Islet-1 was detected by Immunofluorescence cytochemistry. The protein extraction time was determined by Western-blot according to expression levels of Islet-1 -immunoprecipitation. The Islet-1-binding protein was precipitated by co-immunoprecipita-tion (Co-IP). The proteins of HATs and HDACs interacting with Islet-1 were verified by Western-blot. Results Cells in induction group showed the appearance of cardiomyocyte-like cells. The location of Islet-1 was located in cytoplasm. The expression of protein in induction group was significant in the third week (0. 782 ±0. 015) compared with the first 2 weeks. The expression of Islet-1 in induction group was significantly higher than that in blank control group and C3H10 group (0. 819 ± 0. 026,0. 127 ± 0. 006 and 0. 126 ± 0. 001 respectively, P < 0.05). The proteins interacting with Islet-1 were GCN5 ^P300/CBP and HDAC4. Conclusions The synergism of Islet-1 and its interacting proteins such as GCN5、P300/CBP and HDAC4 played an important role in the differentiation of C3H10T1/2 cells into cardiomyocyte-like cells.关键词
C3H10T1/2细胞/Islet-1/心肌样细胞/组蛋白乙酰化Key words
C3H10T1/2 cells/ Islet-1/ cardiomyocyte-like cells/ histone acetylation分类
生物科学引用本文复制引用
林建萍,田杰,刘官信,鲁荣,刘建平,智深深,朱静..Islet-1在乙酰化调控网络中特异性辅助C3H10T1/2细胞向心肌样细胞分化[J].基础医学与临床,2012,32(4):363-368,6.基金项目
国家自然科学基金(No.30973219) （No.30973219）
重庆市自然科学基金(No.2009BA5048) （No.2009BA5048）
