基础医学与临床2012，Vol.32Issue(4)：375-380,6.
体外模拟肿瘤微环境中致大鼠MSCs瘤化因子分析
The analysis of the factors which induced rat mesenchymal stem cells tumorigenesis in vitro simulated tumor microenvironment
摘要
Abstract
Objective To investigate the relation between marrow mesenchymal stem cells tumorigenesis and tyro-sine kinase signaling molecules such as HGF^IL-6,BFGF in vitro. Methods The tumor microenvironment was simulated by co-culturing MSCs with C6 and the normal microenvironment was simulated by co-culturing MSCs with as-trocytes; MSCs were divided into three groups; Experimental group ( co-cultured with C6 ) , Control group ( co-cultured with astrocytes) Blank control group ( MSCs alone) ;P53 and MDM2 of MSCs in each group were measured by PCR, Western blotting; HGF, IL-6 and BFGF expression in each group were detected by ELISA; the STAT3 protein expression of MSCs treated with corresponding ELISA amount tyrosine kinase signaling molecule were detected by immunofluorescence. Results Experimental group cells showed tumor cell-like morphology. MDM2 mRNA in experimental group was the control group 1. 56 ± 0. 21 fold ( P＜0. 05 ) ; MDM2 protein in experimental group increased 90% ±7% as compared to control group (P＜0. 01) ;P53 mRNA decreased to 0. 55 ±0. 10 and the level of mutant P53 protein increased to 87% ±5% in experimental group as compared to that in control group (P＜0. 01); HGF and IL-6 significantly increased in Experimental group as compared to Control group (P＜ 0. 05 ). P-STAT3 expression increased in IL-6 overdose group as compared to IL-6 normaldose group. (P＜0. 01). Conclusion IL-6 can induce mesenchymal stem cells tumorigenesis in vitro simulated tumor microenvironment.关键词
肿瘤微环境/骨髓间充质干细胞/信号传导和转录活化因子3Key words
tumor microenvironment/ mesenchymal stem cells/ STAT3分类
医药卫生引用本文复制引用
刘建平,朱静,田杰,刘官信,林建萍,鲁荣..体外模拟肿瘤微环境中致大鼠MSCs瘤化因子分析[J].基础医学与临床,2012,32(4):375-380,6.基金项目
国家自然科学基金(3114035) （3114035）
重庆市自然科学基金(cstc2011jjA10060) （cstc2011jjA10060）
