基础医学与临床2012，Vol.32Issue(4)：402-406,5.
幽门螺杆菌诱导人胃上皮细胞系(GES-1)发生自噬
Helicobacter pylori induces autophagy of human gastric epithelium cell line(GES-1)
摘要
Abstract
Objective To observe autophagy induced by H. Pylori infection and its modulation in human gastric epithelium ( GES-1). Methods GES-1 was infected by H. Pylori for different hours with the optimal multiplicity of infection ( m. O. I) which was determined by neutral red uptake method ( NRU) . Western blot was utilized for detecting the level of LC3-I/II conversion and P62, which indicated the autophagy induction. The formations of autopha-gosomes were observed via immunofluorescence with microscopy. After the GES-1 infected by H. Pylori were treated with 3MA, E64d and pepstatin A, the changes of autophagy were detected by the methods above. Results The optimum m. O. I was 400 for GES-1 infected by H. Pylori (22695). At 2 h p. I, the autophagy induction became detectable. It reached a peak level at 8 h p. I, while it returned to the basic level at 24 h p. I. 3MA was able to inhibit autophagy with obviously decrease of autophagosome formation. And E64d and pepstatin A could block autophagy downstream to some extent, which is showed by the aggregation of LC3 and P62. Conclusions H. Pylori is capable of inducing autophagy of human gastric epithelium ( GES-1) , and the process can be modulated by autoph-agic regulators.关键词
幽门螺杆菌/自噬/人胃上皮细胞Key words
Helicobacter pylori/ autophagy/ human gastric epithelium分类
医药卫生引用本文复制引用
熊励晶,李佩瑾,童煜,毛萌..幽门螺杆菌诱导人胃上皮细胞系(GES-1)发生自噬[J].基础医学与临床,2012,32(4):402-406,5.基金项目
长江学者和创新团队发展计划(IRT0935) （IRT0935）
