基础医学与临床2012，Vol.32Issue(4)：407-412,6.
川芎嗪对大鼠急性脊髓损伤模型NF-κB及I-κBα表达的影响
The effect of tetramethylpyrazine on the expression of NF-KB and I-KBa after acute spinal cord injury in the rat model
摘要
Abstract
Objective To study the effect of Teramethylpyrazine (TMP) on expression of NF-kB and I-kBo: in a-cute spinal cord injury (ASCI) in rats. Methods Allen'S weight drop method was used to establish acute spinal cord injury (SCI) rat model at T10 section and was conducted in 110 adult Sprague Dawley rats that were subsequently divided into sham operation group (group S, n = 10) ;control group (group C,ti =50) ;Tramethylpyrazine group ( group T, n - 50 ). The function of spinal cord was evaluated with animal behavioral scores by measuring modified Rivilin loxotic plate test and counting BBB and CBS score at 1,3 ,5 ,7,14 and 21 days postoperatively. The injured spinal cord samples were harvested at 1 ,3,6,12 hours, 1 ,3,5,7,14 and 21 days postoperatively(n =5 foreach time point) for the preparation of continuous histological sections that were analyzed the expression of NF-kB and I-kBo: by immunohistochemistry method. Results Group T had significantly higher degrees than group C at 7, 14 and 21 days in modified Rivlin loxotic plate test after SCI (P＜0. 05) ; Group T had significantly higher scores in BBB score than group C at 5,7,14 and 21 days after SCI respectively (P＜0. 05). Group C had significantly higher scores in CBS score than group T at 5 ,7,14 and 21 days after SCI respectively( P＜0. 05). The number of NF-kB positive cells were significantly lower in group T than group C at 1,3 ,5 and 7 days after SCI(P＜0. 05) ; The number of I-kBα: positive cells were significantly lower in group C than group T at 1,3,5 and 7 days after SCI ( P＜ 0. 05). Conclusions The TMP can promote repairing of injured spinal cord tissue through improving the I-kBα expression and decreasing the NF-kB expressions in cells after ASCI.关键词
脊髓损伤/川芎嗪/核因子-kappaB/抑制性卡巴蛋白Key words
spinal cord injury/ tetramethylpyrazine/ NF-kB/ I-kBα分类
医药卫生引用本文复制引用
肖志满,胡建中,吕红斌,卓祥龙,徐大启,王圣轩,黎俊豪..川芎嗪对大鼠急性脊髓损伤模型NF-κB及I-κBα表达的影响[J].基础医学与临床,2012,32(4):407-412,6.基金项目
湖南省自然科学基金(09JJ3047) （09JJ3047）
湖南省卫生厅科研基金(B2007037) （B2007037）
