基础医学与临床2012，Vol.32Issue(4)：433-436,4.
胰腺癌血清差异蛋白表达谱
Serum proteome profiling of pancreatic adenocarcinoma
摘要
Abstract
Objective To identify biomarkers involved in pancreatic adenocarcinoma by serum proteome fingerprinting. Methods ProteoMiner beads were used to treat neat serum sample of pancreatic cancer and control tissues followed by 2-D gel electrophoresis and ESI-MS/MS to identify the signature proteins involved in pancreatic carcino-genesis. ELISA was used to determine serum level of clusterin. Results Seven proteins including clusterin, vitron-ectin, complement C4-A, vitamin D-binding protein, anitthrombin- M , complement component C and prothrombin were up-regulated and complement component C7 was down-regulaed in serum of pancreatic cancer. ELISA results showed that serum level of clusterin enhanced in pancreatic adenocarcinoma significantly ( P＜0. 05). Conclusions Clusterin may correlate closely with development and progression of pancreatic adenocarcinoma and serum level of clusterin may serve as a key biomarker for diagnosis of pancreatic adenocarcinoma.关键词
胰腺癌/高丰度蛋白/低丰度蛋白/凝集素/补体Key words
pancreatic adenocarcinoma/ high-abundance proteins/ low-abundance proteins/ clusterin/ complement分类
医药卫生引用本文复制引用
周金桥,刘秋红,高景,宋来君..胰腺癌血清差异蛋白表达谱[J].基础医学与临床,2012,32(4):433-436,4.基金项目
河南省医学科技攻关项目(2011020017) （2011020017）
