基础医学与临床2012，Vol.32Issue(4)：447-450,4.
Rh血型弱D和DEL表型的基因突变分析
Gene mutation analysis of the Rh weak D and DEL phenotype
金辉 1张一兵 1佟青 1罗喜钢1
作者信息
- 1. 辽宁医学院附属三院检验中心,辽宁锦州121000
- 折叠
摘要
Abstract
Objective To investigate the gene mutation basis of the Rh weak D and DEL phenotype. Methods Weak D and DEL phenotype expression were screened out with an indirect anti-human globulin test(IAT) and absorption elution test. Then the nucleotide sequences of ten exons of weak D and DEL phenotype were evaluated by a RHD gene-specific PCR-SSP and sequencing. Results The results showed that weak D phenotype harbored a RHD 845 G＞A mutation in exon 6, Rh DEL phenotype harbored a RHD 1227G＞A mutation in exon 9. Conclusions The RHD gene sequencing method is established, the molecular basis of weak D and DEL phenotype is characterized, and this study is the basis for discovering the new D variation phenotypes and new RHD alleles.关键词
RHD基因/弱D表型/DEL表型/测序Key words
RHD gene/ weak D phenotype/ DEL phenotype/ sequencing分类
医药卫生引用本文复制引用
金辉,张一兵,佟青,罗喜钢..Rh血型弱D和DEL表型的基因突变分析[J].基础医学与临床,2012,32(4):447-450,4.
