寄生虫与医学昆虫学报2012，Vol.19Issue(1)：8-11,4.DOI:10.3969/j.issn.1005-0507.2012.01.002
卡介苗接种对小鼠伯氏疟原虫感染早期树突状细胞活化状态的影响
EFFECTS OF BCG VACCINATION ON DENDRITIC CELL ACTIVATION OF MICE IN EARLY STAGE OF INFECTION WITH PLASMODIUM BERGHEI
王各各 1扬帆 1刘军 1李莹 1延娟 1曹雅明1
作者信息
- 1. 中国医科大学基础医学院免疫学教研室,沈阳,110001
- 折叠
摘要
Abstract
To investigate the BCG vaccination on dendritic cell ( DC ) activation of mice in the early stage of infection with Plasmodium spp. , C57BL/6 mice were infected by intraperitoneal injection of 110 P. Berghei parasitized in erythrocytes, then injected with BCG on day 3 after infection ( P. B-3-B group ), the mice only infected with P. Berghei without BCG injection served as control group. The level of parasitemia was observed by means of Giemsa staining of thin blood smears, and applying flow cytometry to quantitively analyze the percentages of CDllc+ CDllb+ DC, CDllc+ B220+ DC, CDllc+ CD80+ DC and CDllc+ MHC II + DC in spleen cells on day 0, 5 and 8 after infection. It was found that, the parasitemia of P. B-3-B group gradually increased from day 8 after infection, 66. 7% of the mice survived the parasitemia until day 19 and died at day 20. Mice in control group died of cerebral malaria on 8 ~ 10 days. The percentages of CDllc+ CDllb+ DC, CDllc+ CD80+ DC and CDllc+ MHC II + DC decreased significantly in P. B-3-B group than in control group ( P <0. 05 or P <0. 01 ) on day 5 and 8 after infection, and the percentage of CDllc+ B220+ DC was also decreased significantly in P. B-3-B group than in control group ( P <0. 01 ) on day 8 after infection. These results suggested that BCG vaccination in the early stage of malaria infection may reduce the percentage of DC subsets and the expression of DC surface molecules, thereby influence the progress of malaria infection.关键词
脑型疟疾/伯氏疟原虫/卡介苗/树突状细胞Key words
Cerebral malaria/ Plasmodium berghei ANKA/ Bacillus Calmette-Guerin/ Dendritic cell引用本文复制引用
王各各,扬帆,刘军,李莹,延娟,曹雅明..卡介苗接种对小鼠伯氏疟原虫感染早期树突状细胞活化状态的影响[J].寄生虫与医学昆虫学报,2012,19(1):8-11,4.
