寄生虫与医学昆虫学报2012，Vol.19Issue(1)：12-18,7.DOI:10.3969/j.issn.1005-0507.2012.01.003
白纹伊蚊实验种群感染沃尔巴体Wolbachia的研究
STUDY ON WOLBACHIA INFECTION IN EXPERIMENTAL POPULATION OF AEDES ALBOPICTUS
张东京 1杨潇 1吴贤生 1陈婧 1李卓雅 1吴瑜 1詹希美 1郑小英1
作者信息
- 1. 中山大学中山医学院寄生虫教研室,中山大学热带病研究教育部重点实验室,广州,510080
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摘要
Abstract
In order to investigate the infection profiles of Wolbachia in Aedes albopictus rearing in laboratory, we carried out a survey in Ae. Albopictus using polymerase chain reaction ( PCR ) assay to detect Wolbachia to analysis the infection rates, strains, tissue distribution and the phylogeny relationships. Briefly, template DNA was extracted from the head, ovary/ testis, fat body, salivary glands/ thorax, malpighian tubules and midgut respectively to amplify Wolbachia Surface Protein ( wsp ) gene. The amplification product was cloned and sequenced to analyze the phylogenesis with BLAST tools. Of fifty male and female mosquitoes randomly selected, all of them were positively infected with Wolbachia. The ovary/tesis, fat body and salivary glands/thorax of both sexes were superinfected by Wolbachia A and B strains. The malpighian tubules and midgut of male were only infected by B strain. As for the head, the female were found only infected by B strain, the male were not found infection. The identity of Wolbachia in Ae. Albopictus, is 99% as high as Culex pipents, Cotesia sesamiae and Eurema necabe, which suggests that Wolbachia in Ae. Albopictus, Culex pipents, Cotesia sesamiae and Eurema necabe may originate from the same subgroup.关键词
沃尔巴体Wolbachia/白纹伊蚊/表面膜蛋白(wsp)/系统发育Key words
Wolbachia/ Aedes albopictus/ Wsp/ Phylogenesis引用本文复制引用
张东京,杨潇,吴贤生,陈婧,李卓雅,吴瑜,詹希美,郑小英..白纹伊蚊实验种群感染沃尔巴体Wolbachia的研究[J].寄生虫与医学昆虫学报,2012,19(1):12-18,7.
